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UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION IN INTERPLOIDY CROSSES WITH ‘ORAH’ MANDARIN (CITRUS RETICULATA)

Frontiers of Agricultural Science and Engineering 2021, Volume 8, Issue 2,

Abstract: By analyzing their genetic origins using nine novel single nucleotide polymorphism (SNP) markers, allThese tetraploid plantlets were genotyped using eight pericentromeric SNP markers and nine centromeredistal SNP markers.Based on the genotypes of the 2n megagametophytes, the parental heterozygosity rates in 16 SNP loci and

Keywords： Citrus / 2n gamete / interploidy hybridization / pericentromeric SNP marker / second division restitution    

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UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION IN INTERPLOIDY CROSSES WITH ‘ORAH’ MANDARIN (

Qiangming XIA, Wei WANG, Kaidong XIE, Xiaomeng WU, Xiuxin DENG, Jude W. GROSSER, Wenwu GUO

Frontiers of Agricultural Science and Engineering   Pages 302-313 doi: 10.15302/J-FASE-2021385

Abstract: By analyzing their genetic origins using nine novel single nucleotide polymorphism (SNP) markers, allThese tetraploid plantlets were genotyped using eight pericentromeric SNP markers and nine centromeredistal SNP markers.Based on the genotypes of the 2n megagametophytes, the parental heterozygosity rates in 16 SNP loci and

Keywords： Citrus     2n gamete     interploidy hybridization     pericentromeric SNP marker     second division restitution    

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Transcriptome resources and genome-wide marker development for Japanese larch (

Wanfeng LI,Suying HAN,Liwang QI,Shougong ZHANG

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 1,   Pages 77-84 doi: 10.15302/J-FASE-2014010

Abstract: While the differential responses of trees to changes in climatic and environmental conditions have been demonstrated as they age, the underlying mechanisms and age control of tree growth and development are complex and poorly understood particularly at a molecular level. In this paper, we present a transcriptome analysis of , a deciduous conifer that is widely-grown in the northern hemisphere and of significant ecological and economic value. Using high-throughput RNA sequencing, we obtained about 26 million reads from the stems of 1-, 2-, 5-, 10-, 25- and 50-year-old trees. Combining these with the published Roche 454 sequencing reads and the expressed sequence tags (both mainly from embryogenic cell cultures), we assembled 26670549 reads into 146786 transcripts, of which we annotated 79182 to support investigations of the molecular basis of tree aging and adaption, somatic embryogenesis and wood formation. Using these sequences we also identified many single-nucleotide polymorphisms, simple sequence repeats, and insertion and deletion markers to assist breeding and genetic diversity studies of .

Keywords： Larix     transcriptome     age     wood formation     somatic embryogenesis     molecular marker    

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Chinmedomics facilitated quality-marker discovery of Sijunzi decoction to treat spleen qi deficiency

Qiqi Zhao, Xin Gao, Guangli Yan, Aihua Zhang, Hui Sun, Ying Han, Wenxiu Li, Liang Liu, Xijun Wang

Frontiers of Medicine 2020, Volume 14, Issue 3,   Pages 335-356 doi: 10.1007/s11684-019-0705-9

Abstract: A total of 58 biomarkers were related to SQDS, and SJZD can adjust a large proportion of marker metabolitesisoglabrolide, and glycyrrhetnic acid as the q-markers of licorice; and 2-atractylenolide as the q-marker

Keywords： traditional Chinese medicine     Sijunzi decoction     spleen qi deficiency syndrome     Chinmedomics     quality-marker    

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Development of a 50K SNP Array for Japanese Flounder and Its Application in Genomic Selection for Disease Article

Qian Zhou, Ya-dong Chen, Sheng Lu, Yang Liu, Wen-teng Xu, Yang-zhen Li, Lei Wang, Na Wang, Ying-ming Yang, Song-lin Chen

Engineering 2021, Volume 7, Issue 3,   Pages 406-411 doi: 10.1016/j.eng.2020.06.017

Abstract:

Single nucleotide polymorphism (SNP) arrays are a powerful genotyping tool used in genetic researchWe developed a 50K Japanese flounder SNP array, "Yuxin No. 1," and report its utility in genomicbest linear unbiased prediction (ABLUP), suggesting good performance of the "Yuxin No. 1" SNPIn summary, we developed the "Yuxin No. 1" 50K SNP array, which provides a useful platform

Keywords： 牙鲆     单核苷酸多态性     SNP芯片     抗病性     基因组选择    

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Protein phosphatase magnesium-dependent 1δ is a novel tumor marker and target in hepatocellular carcinoma

Zhi Xu,Chunxiang Cao,Haiyan Xia,Shujing Shi,Lingzhi Hong,Xiaowei Wei,Dongying Gu,Jianmin Bian,Zijun Liu,Wenbin Huang,Yixin Zhang,Song He,Nikki Pui-Yue Lee,Jinfei Chen

Frontiers of Medicine 2016, Volume 10, Issue 1,   Pages 52-60 doi: 10.1007/s11684-016-0433-3

Abstract:  Overall, results suggest that PPM1D is a potential prognostic marker and therapeutic target

Keywords： PPM1D     hepatocellular carcinoma     prognosis     target therapy    

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Novel polymorphic EST-based microsatellite marker isolation and characterization from

Manosh Kumar BISWAS,Peng CHEN,Mohamed Hamdy AMAR,Xiuxin DENG

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 1,   Pages 60-65 doi: 10.15302/J-FASE-2015048

Abstract: Novel simple sequence repeat (SSR) markers were developed to evaluate their utility for genetic diversity and breeding studies of and related species. A total of 108 primer pairs were characterized by PCR amplification experiments. Among these, 61 were polymorphic and transferable to other citrus species. The number of alleles per locus ranged from 2 to 6, with an average of 2.37 alleles per locus. The expected heterozygosity and observed heterozygosity ranged from 0 to 0.83 and 0 to 1.00, respectively. These novel polymorphic SSR markers will be useful for citrus cultivar identification and evaluation as well as breeding studies.

Keywords： microsatellite     polymorphism     Poncirus trifoliata    

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Erratum to “Targeted Genotyping of a Whole-Gene Repertoire by an Ultrahigh-Multiplex and Flexible HD-Marker Erratum

Pingping Liu,Jia Lv,Cen Ma,Tianqi Zhang,Xiaowen Huang,Zhihui Yang,Lingling Zhang,Jingjie Hu,Shi Wang,Zhenmin Bao,

Engineering 2022, Volume 18, Issue 11,   Pages 259-259 doi: 10.1016/j.eng.2022.09.002

Keywords： online     THE AUTHORS.Published     repertoire     behalf     Engineering     September     genotyping     Academy     HD-marker approach    

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Atomic Interface Catalytically Synthesizing SnP/CoP Hetero-Nanocrystals within Dual-Carbon Hybrids for Article

Chen Hu,Yanjie Hu,Aiping Chen,Xuezhi Duan,Hao Jiang,Chunzhong Li,

Engineering 2022, Volume 18, Issue 11,   Pages 154-160 doi: 10.1016/j.eng.2021.11.026

Abstract: Herein, an in situ catalytic phosphorization strategy is developed to synthesize SnP/CoP hetero-nanocrystalswithin reduced graphene oxide (rGO)-coated carbon frameworks, in which the SnP relative formation energyThe lithiation/delithiation mechanism is disclosed, showing that the 4.0 nm sized SnP/CoP nanocrystalsformed metallic Co of CoP at a relatively high potential accelerates the subsequent reaction kinetics of SnP

Keywords： Catalytic phosphorization     SnP     Hetero-nanocrystals     Fast charging     Li-ion batteries    

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Detection of genomic signatures for pig hairlessness using high-density SNP data

Ying SU,Yi LONG,Xinjun LIAO,Huashui AI,Zhiyan ZHANG,Bin YANG,Shijun XIAO,Jianhong TANG,Wenshui XIN,Lusheng HUANG,Jun REN,Nengshui DING

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 4,   Pages 307-313 doi: 10.15302/J-FASE-2014039

Abstract: conducted a genomic scan for genetically differentiated regions between hairy and hairless pigs using 60K SNP

Keywords： hairlessness     pig     selective sweeping    

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as proxies for yield potential and heat stress tolerance in spring wheat: heritability estimates and marker-trait

Caiyun LIU, Francisco PINTO, C. Mariano COSSANI, Sivakumar SUKUMARAN, Matthew P. REYNOLDS

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 3,   Pages 296-308 doi: 10.15302/J-FASE-2019269

Abstract: using 18704 single nucleotide polymorphisms (SNPs) from Illumina iSelect 90K identified 280 and 43 marker-traitHigh heritability estimates and identification of marker-trait associations indicate that SRIs are useful

Keywords： genome-wide association study (GWAS)     heat tolerance     spectral reflectance     spring wheat    

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A genome-wide association study of five meat quality traits in Yorkshire pigs

Qian DONG,Huiying LIU,Xinyun LI,Wei WEI,Shuhong ZHAO,Jianhua CAO

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 2,   Pages 137-143 doi: 10.15302/J-FASE-2014014

Abstract: drip loss within 24 h and water-holding capacity in 231 Yorkshire barrows using illumina porcine 60k SNPThe results showed that a total of 344 single nucleotide polymorphisms (SNP) were significantly associatedIn addition, 25 haplotype blocks based on 116 SNPs were revealed with SNP combination patterns for fiveOur study added new SNP information for identification of meat quality traits in pigs and will help elucidate

Keywords： Pig     GWAS     meat quality trait     SNP    

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The genetic regulation of skeletal muscle development: insights from chicken studies

Wen LUO, Bahareldin A. ABDALLA, Qinghua NIE, Xiquan ZHANG

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 3,   Pages 295-304 doi: 10.15302/J-FASE-2017159

Abstract: Skeletal muscle development is a complex multi-process trait regulated by various genetic factors. The chicken embryo is an ideal model system for studying skeletal muscle development. However, only a small proportion of the genetic factors affecting skeletal muscle development have been identified in chicken. The aim of this review is to summarize recent knowledge about the genetic factors involved in the regulation of skeletal muscle development in the chicken, such as gene polymorphisms, epigenetic modification, noncoding RNAs and transcription factors, which can influence skeletal muscle development at the genome, epigenome, transcriptome and proteome levels. Research on the regulation of skeletal muscle development in chicken is not yet comprehensive and most of the candidate genes and single nucleotide polymorphisms related to chicken muscle growth remain to be verified in experimental studies. In addition, the data derived from transcriptome sequencing and genome-wide association studies still require further investigation and analysis and comprehensive studies on the regulation of chicken skeletal muscle development will continue as a major research focus.

Keywords： chicken     epigenetic modification     miRNAs     skeletal muscle development     SNP     transcription factor    

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The microRNA, miR-29c, participates in muscle development through targeting the

Weiya ZHANG,Wei WEI,Yuanyuan ZHAO,Shuhong ZHAO,Xinyun LI

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 4,   Pages 311-317 doi: 10.15302/J-FASE-2015075

Abstract: Previous studies indicated that miR-29c is important for muscle development in mice and human, but its role in pigs is unknown. In this study, we detected the expression of miR-29c in Meishan longissimus lumborum (LL) muscle. The results showed that miR-29c was gradually upregulated during development of skeletal muscle in pig. Moreover, the expression of and genes, which were confirmed to be targeted by miR-29c in mice, was decreased along with muscle development. Furthermore, the expression level of miR-29c was significantly higher in adult Meishan pigs than Large White pigs, while the expression of and genes was significantly lower in Meishan pigs. These results indicated that the expression pattern of miR-29c was opposite to that of and genes in pigs. Also, the luciferase assay indicated that miR-29s can target the gene in pigs. In addition, we identified a T to C mutation in the primary transcript of miR-29c, which was associated with the postmortem muscle pH in pigs. Based on these results, we concluded that miR-29c is also important in skeletal muscle development of pigs.

Keywords： pig     miR-29c     skeletal muscle     expression     SNP    

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Genome-wide association study of the backfat thickness trait in two pig populations

Dandan ZHU,Xiaolei LIU,Rothschild MAX,Zhiwu ZHANG,Shuhong ZHAO,Bin FAN

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 2,   Pages 91-95 doi: 10.15302/J-FASE-2014005

Abstract: identified in two independent pig populations, using a genome-wide association study of porcine 60K SNPFor each population, 30 most significant single-nucleotide polymorphisms (SNPs) were selected and SNPThe most significant SNP (ALGA0006623) was located on SSC1, upstream of the gene.

Keywords： backfat thickness     SNP chip     genome-wide association study     compressed mixed linear model     pig    

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Title Author Date Type Operation

UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION IN INTERPLOIDY CROSSES WITH ‘ORAH’ MANDARIN (CITRUS RETICULATA)

Journal Article

UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION IN INTERPLOIDY CROSSES WITH ‘ORAH’ MANDARIN (

Qiangming XIA, Wei WANG, Kaidong XIE, Xiaomeng WU, Xiuxin DENG, Jude W. GROSSER, Wenwu GUO

Journal Article

Transcriptome resources and genome-wide marker development for Japanese larch (

Wanfeng LI,Suying HAN,Liwang QI,Shougong ZHANG

Journal Article

Chinmedomics facilitated quality-marker discovery of Sijunzi decoction to treat spleen qi deficiency

Qiqi Zhao, Xin Gao, Guangli Yan, Aihua Zhang, Hui Sun, Ying Han, Wenxiu Li, Liang Liu, Xijun Wang

Journal Article

Development of a 50K SNP Array for Japanese Flounder and Its Application in Genomic Selection for Disease

Qian Zhou, Ya-dong Chen, Sheng Lu, Yang Liu, Wen-teng Xu, Yang-zhen Li, Lei Wang, Na Wang, Ying-ming Yang, Song-lin Chen

Journal Article

Protein phosphatase magnesium-dependent 1δ is a novel tumor marker and target in hepatocellular carcinoma

Zhi Xu,Chunxiang Cao,Haiyan Xia,Shujing Shi,Lingzhi Hong,Xiaowei Wei,Dongying Gu,Jianmin Bian,Zijun Liu,Wenbin Huang,Yixin Zhang,Song He,Nikki Pui-Yue Lee,Jinfei Chen

Journal Article

Novel polymorphic EST-based microsatellite marker isolation and characterization from

Manosh Kumar BISWAS,Peng CHEN,Mohamed Hamdy AMAR,Xiuxin DENG

Journal Article

Erratum to “Targeted Genotyping of a Whole-Gene Repertoire by an Ultrahigh-Multiplex and Flexible HD-Marker

Pingping Liu,Jia Lv,Cen Ma,Tianqi Zhang,Xiaowen Huang,Zhihui Yang,Lingling Zhang,Jingjie Hu,Shi Wang,Zhenmin Bao,

Journal Article

Atomic Interface Catalytically Synthesizing SnP/CoP Hetero-Nanocrystals within Dual-Carbon Hybrids for

Chen Hu,Yanjie Hu,Aiping Chen,Xuezhi Duan,Hao Jiang,Chunzhong Li,

Journal Article

Detection of genomic signatures for pig hairlessness using high-density SNP data

Ying SU,Yi LONG,Xinjun LIAO,Huashui AI,Zhiyan ZHANG,Bin YANG,Shijun XIAO,Jianhong TANG,Wenshui XIN,Lusheng HUANG,Jun REN,Nengshui DING

Journal Article

as proxies for yield potential and heat stress tolerance in spring wheat: heritability estimates and marker-trait

Caiyun LIU, Francisco PINTO, C. Mariano COSSANI, Sivakumar SUKUMARAN, Matthew P. REYNOLDS

Journal Article

A genome-wide association study of five meat quality traits in Yorkshire pigs

Qian DONG,Huiying LIU,Xinyun LI,Wei WEI,Shuhong ZHAO,Jianhua CAO

Journal Article

The genetic regulation of skeletal muscle development: insights from chicken studies

Wen LUO, Bahareldin A. ABDALLA, Qinghua NIE, Xiquan ZHANG

Journal Article

The microRNA, miR-29c, participates in muscle development through targeting the

Weiya ZHANG,Wei WEI,Yuanyuan ZHAO,Shuhong ZHAO,Xinyun LI

Journal Article

Genome-wide association study of the backfat thickness trait in two pig populations

Dandan ZHU,Xiaolei LIU,Rothschild MAX,Zhiwu ZHANG,Shuhong ZHAO,Bin FAN

Journal Article